Chromatography

HPLC

Troubleshooting HPLC Method Development Issues

High-Performance Liquid Chromatography (HPLC) is a widely used analytical technique, offering precise separation and quantification of compounds in a sample. However, method development in HPLC is not always a straightforward process. Analysts often encounter various issues that can compromise the quality of results. In this article, we will delve into common problems that arise during […]

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HPLC

HPLC Detectors: Types and Applications

High-Performance Liquid Chromatography (HPLC) is a powerful analytical technique used in various fields, including pharmaceuticals, environmental analysis, food quality control, and more. HPLC detectors are integral components of the system, responsible for detecting and quantifying the compounds separated in the chromatographic column. In this article, we delve into the diverse world of HPLC detectors, exploring

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HPLC

Gradient vs. Isocratic HPLC: When to Use Which?

High-Performance Liquid Chromatography (HPLC) is a versatile analytical technique used for separating and quantifying compounds within a sample. A fundamental decision that analysts face when developing an HPLC method is whether to employ a gradient elution or an isocratic elution. Both methods have their merits and are suited to different applications. In this article, we

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HPLC

HPLC Method Development: A Beginner’s Comprehensive Guide | ICH Guidelines & Practical Insights

Explore the intricacies of HPLC Method Development with this comprehensive beginner’s guide. Understand the principles, methodologies, and practical applications essential for navigating the world of High-Performance Liquid Chromatography. Dive into ICH guidelines and foundational concepts to grasp the complexities of HPLC methodology for effective analytical separation

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