Gradient vs. Isocratic HPLC: When to Use Which?
High-Performance Liquid Chromatography (HPLC) is a versatile analytical technique used for separating and quantifying compounds within a sample. A fundamental decision that analysts face when developing an HPLC method is whether to employ a gradient elution or an isocratic elution. Both methods have their merits and are suited to different applications. In this article, we […]
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